endolysosomal

With the data of the present report, however, endolysosomal membranes can now potentially be included in regulatory activity mediated by Irgm1.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

In future analyses it should be borne in mind that tagging of Irgm1 leads to loss of Golgi localisation and enhanced localisation on endolysosomal membranes, probably as a result of constitutive activation.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

The targeting motif known to be required for Golgi localisation is surprisingly also required for endolysosomal localisation.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

However, unlike Golgi localisation, localisation to the endolysosomal system also requires the functional integrity of the nucleotide binding site, and thus probably reflects transient activation.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

Irgm1 is the first IRG protein to be found associated with the endolysosomal membrane system in addition to either Golgi (Irgm1 and Irgm2) or ER (Irgm3) membranes, and we interpret the result to be in favour of a regulatory function of IRGM proteins at cellular membrane systems.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

Golgi localisation is lost when Irgm1 is tagged at either N - or C-termini with EGFP, while localisation to the endolysosomal system is relatively favoured.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

Irgm1 has thus a constitutive association with the endolysosomal membrane system in addition to the Golgi apparatus, a unique property among IRG proteins tested so far.

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010

Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, et al. (2006) A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway.

PLoS ONE Alerts: New Articles 2009