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Examples
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Our longstanding belief that enolpyruvate is the intermediate in the pyruvate kinase reaction was confirmed by direct chemical analysis of pyruvate kinase in the steady state41.
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Heavy atom NMR spectroscopy of the hydrogens of P-enolpyruvate led to Mildred Cohn's solution of the reaction stereochemistry of the reaction of enolase using (3R) - phosphoglyceric acid-3-d39.
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We could also show that enolpyruvate synthesized by treating PEP with a phosphatase was used by pyruvate kinase at an appropriate rate although its affinity was less than expected for an intermediate42.
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