flowcytometric

In the recent MRD-AML-BFM Group study (the first European flowcytometric MRD study in childhood AML),

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Sievers et al. 3 showed, in a retrospective flowcytometric MRD study, that the relative risk of relapse in MRD-positive pediatric AML patients was 2.8 times the risk of relapses in MRD-negative patients.

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This four-color flowcytometric study detected a leukemia-associated immunophenotype (LAIP) in 85 % of patients with sensitivities of 0. 1-0.01 % using patient-tailored labelings.

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In conclusion, flowcytometric MRD detection is possible in children with AML.

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In a more recent study from the same group, using a comparable flowcytometric approach and the same cut-off level of 0.5 %, patients with occult leukemia after induction therapy were almost five times more likely to relapse than those lacking detectable MRD.

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On arrival, samples were analyzed immediately for flowcytometric immunophenotyping; remaining cells were subjected to Ficoll density centrifugation and RNA was isolated from the mononuclear cells.

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Clinical significance of flowcytometric minimal residual disease detection in pediatric acute myeloid leukemia patients treated according to the DCOG ANLL97

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AML, but that optimal time points (TPs) and cut-off levels are dependent on the flowcytometric methodology and on the treatment protocol.

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For flowcytometric analysis, CD3+ were kept on ice and washed extensively with citrated PBS containing 1% FCS. 10 µl of FITC, APC or PE - conjugated antibody was added and incubated on ice for 20 min. 300 µl FACS buffer was added and T cells were pelleted, resuspended in 200 µl buffer.

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4 A flowcytometric study by Coustan-Smith et al. 1 reported pediatric AML patients with MRD levels

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