fluoresceinated

Engrafted HH in paraffin embedded liver sections were identified using the Super Sensitive ISH Detection System (Biogenex, San Ramon, CA) with fluoresceinated Alu probe (cat # HK844-2K, Biogenex, San Ramon, CA) according to the manufacturer's instructions.

PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

Engrafted human hepatocytes (HH, brown nuclei) in paraffin embedded liver sections were distinguished from mouse hepatocytes (MH, blue nuclei) and other murine constituents (M, blue nuclei) by hybridization with fluoresceinated Alu probe (i, left).

PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

Monolayers were incubated with rabbit SPAK antibody and then with relevant fluoresceinated secondary antibody same as for colon cryostat sections.

PLoS ONE Alerts: New Articles 2009

Briefly, 7-μm cryostat sections of mouse colon were fixed in buffered 4\% paraformaldehyde for 30 min, blocked specimen in 5\% normal rabbit serum in PBS / Triton for 1 h, and incubated with rabbit SPAK antibody (Cell signalling technology Inc, Danvers, MA) overnight at 4°C, washed with phosphate-buffered saline (PBS), and subsequently incubated with fluoresceinated secondary antibody for 1 h at room temperature.

PLoS ONE Alerts: New Articles 2009

Table 2) were identified by hybridization with fluoresceinated Alu probe (left).

PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

Table 2) were identified by hybridization with fluoresceinated Alu probe (top).

PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

The two anti-Irgm1 reagents were used to stain primary MEFs induced for 24 hr with 100 U IFNg, followed by appropriate fluoresceinated secondary anti-immunoglobulin reagents (see

PLoS ONE Alerts: New Articles Yang O. Zhao et al. 2010