from The American Heritage® Dictionary of the English Language, 4th Edition
- n. Any of a group of fluorescent dyes used to stain biological specimens.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. Any of various fluorescent dyes used to stain biological material before microscopic examination
from the GNU version of the Collaborative International Dictionary of English
- n. Any of various fluorescent substances used in fluorescence microscopy to stain specimens.
from WordNet 3.0 Copyright 2006 by Princeton University. All rights reserved.
- n. any of various fluorescent substances used in fluorescence microscopy to stain specimens
Sorry, no etymologies found.
There are also three more tables that list several dendritic cell and T cell relevant antigens, the function of the antigen, the fluorochrome available, and the exciting laser.
(DLIT4) images from an IVIS Spectrum optical imaging system, which enables cell number determination of a fluorochrome.
Following development of in situ signal, sections were counterstained with anti-K5 and anti-K8 antibodies followed by fluorochrome conjugated secondary reagents to allow co-localization of Cells were suspended in 100 µl of FACS staining buffer (FSB-1% fetal bovine serum, 5 mM EDTA and 0. 02% NaN 3 in PBS) with appropriately diluted primary antibodies for 20 minutes on ice in the dark.
A mean background plus 5% was used as the minimum threshold for each specific fluorochrome.
Thus the peptide could be loaded onto fluorochrome-conjugated streptavidin protein, which has four biotin binding sites, to form a tetrameric, fluorescent peptide-streptavidin complex.
The cell samples were treated with FcR-blocking reagent (Miltenyi Biotec) for 10 minutes, stained with the fluorochrome-conjugated antibodies for 30 minutes, and washed three times with PBS/10% FCS.
Trimerbodies were labeled with the near-infrared fluorochrome Cy5 and injected in the tail vein of nude mice bearing MKN45 (stomach adenocarcinoma), HT-1080 (fibrosarcoma), or HeLa
Only about 5-10 percent of the emitted light from an excited fluorochrome is collected and transferred to the sensor in a typical epi-fluorescence microscope.
Briefly, following TLR2/1L stimulation, cells were harvested from the culture plate and incubated with the primary monoclonal antibody against IL-1R1 (R&D Systems), then a biotinylated secondary antibody, and finally strep-avidin conjugated with a fluorochrome.
With a plethora of fluorochrome pigments to choose from - including pigments using the fluorescence protein technology for which a Nobel Prize was awarded in 2008 and clinically approved fluorescent agents - observing metabolic and molecular processes in all kinds of living organisms, from fish to mice and humans, will be possible.