formazan

MTT was incubated for 3 hours at 37°C, the medium removed, and the formazan product dissolved in 100 ml DMSO.

PLoS ONE Alerts: New Articles Jamila Khallou-Laschet et al. 2010

Only viable cells reduce WST-1 to produce formazan with the ratio of absorbances at 450 and 630 nm correlated to the number of viable cells in the culture.

PLoS ONE Alerts: New Articles Carlene S. Starck et al. 2010

Only viable cells reduce WST-1 to produce formazan with the ratio of absorbances at 450 and 630 nm correlated to the number of viable cells in the culture.

PLoS ONE Alerts: New Articles Carlene S. Starck et al. 2010

The formazan was solubilized by dimethyl sulfoxide and colorimetric absorbance was quantified by measuring the optical density (OD) at

PLoS ONE Alerts: New Articles Yo-Ping Lai et al. 2009

MTT is especially useful for assaying the quantification of viable cells, because MTT is cleaved to form a formazan dye (purple color) only by metabolic active cells

PLoS ONE Alerts: New Articles Yo-Ping Lai et al. 2009

Soluble formazan formation was determined by absorption measurement at 450 nm.

PLoS ONE Alerts: New Articles 2009

The absorbance at 490 nm of formazan product in transwells supernatants was measured after transfer to a 96-well plate.

PLoS ONE Alerts: New Articles 2009

The assay utilizes a novel tetrazolium compound that metabolically active cells convert to a water-soluble formazan by the action of cellular dehydrogenases, which is measured by absorbance at 490 nm using a colorimetric microtiter plate reader

PLoS ONE Alerts: New Articles Maria Marchetti et al. 2009

The MTS conversion into formazan was realized into transwells according to manufacturer recommandations.

PLoS ONE Alerts: New Articles 2009

The absorbance of the formazan product was measured at a wavelength of 570 nm with 750 nm (subtracted) as reference.

PLoS ONE Alerts: New Articles Branislava Janic et al. 2009