Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. the organic nitrogen compound H2NN=CHN=NH or any of its derivatives

Etymologies

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Examples

  • Only viable cells reduce WST-1 to produce formazan with the ratio of absorbances at 450 and 630 nm correlated to the number of viable cells in the culture.

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  • MTT was incubated for 3 hours at 37°C, the medium removed, and the formazan product dissolved in 100 ml DMSO.

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  • Soluble formazan formation was determined by absorption measurement at 450 nm.

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  • The absorbance at 490 nm of formazan product in transwells supernatants was measured after transfer to a 96-well plate.

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  • The MTS conversion into formazan was realized into transwells according to manufacturer recommandations.

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  • The absorbance of the formazan product was measured at a wavelength of 570 nm with 750 nm (subtracted) as reference.

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  • MTT is especially useful for assaying the quantification of viable cells, because MTT is cleaved to form a formazan dye (purple color) only by metabolic active cells

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  • The formazan was solubilized by dimethyl sulfoxide and colorimetric absorbance was quantified by measuring the optical density (OD) at

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  • The assay utilizes a novel tetrazolium compound that metabolically active cells convert to a water-soluble formazan by the action of cellular dehydrogenases, which is measured by absorbance at 490 nm using a colorimetric microtiter plate reader

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  • The production of superoxide by XO produces the formazan dye, which can be detected by measuring absorbance at 450 nm.

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