glycosidase

Fetuin was resistant to single O-glycosidase digestion due to the fact that its N-linked and O-linked oligosaccharides are sialylated Optineurin is neither N - (A) nor O - (B) glycosylated but is phosphorylated (C).

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

The former shifted from 17 to 15 kDa and the latter from 64 to 55 kDa with O-glycosidase and sialidase treatment.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Cross-contamination of cytosolic proteins into the membrane fraction is typically Total protein (50-100 µg) from RGC5 cells or those transfected with pTarget-FLAG-OPTN was digested with PGNase F (0.25 units, Enzymatic In-Solution N-deglycosylation kit, Sigma) for N-deglycosylation or with O-glycosidase (2.5 mUnits, Sigma) without or with addition of sialidase A (5 mUnits, Sigma) for O-deglycosylation per manufacturer's instructions.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Fetuin was resistant to a single O-glycosidase digestion due to the fact that its N-linked and O-linked oligosaccharides are sialylated.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Kuntz DA, Rose DR, Pinto BM (2005) A combined STD-NMR/molecular modeling protocol for predicting the binding modes of the glycosidase inhibitors kifunensine and salacinol to Golgi alpha-mannosidase II.

PLoS ONE Alerts: New Articles Michael D. L. Suits et al. 2010

Fetuin was resistant to a single O-glycosidase digestion due to the fact that its N-linked and O-linked oligosaccharides are sialylated.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Fetuin was resistant to single O-glycosidase digestion due to the fact that its N-linked and O-linked oligosaccharides are sialylated Optineurin is neither N - (A) nor O - (B) glycosylated but is phosphorylated (C).

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

The former shifted from 17 to 15 kDa and the latter from 64 to 55 kDa with O-glycosidase and sialidase treatment.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Lysates from RGC5 cells were treated with peptide N-glycosidase F (PNGase F, for N-deglycosylation) or O-glycosidase (for O-deglycosylaton) without or with α-2 (3,6,8,9) - neuraminidase (sialidase), and Western blotted for optineurin.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

The natural product swainsonine is known to be a versatile glycosidase inhibitor having previously been used to study the active center of the.

PLoS ONE Alerts: New Articles Michael D. L. Suits et al. 2010