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- noun biochemistry An
enzymethat transfersa guanosine monophosphategroup, usually from GTPto another molecule, releasing pyrophosphate.
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In metazoans a two-component enzyme complex engendering triphosphatase, guanylyltransferase, and methyltransferase enzymatic activities is responsible for modifying RNAs at the 5′ end with m
An in vitro transcribed RNA was capped with guanylyltransferase using [α - 32P] GTP and then incubated with immunopurified intact gemin5 or the gemin5 (Δ25) deletion mutant in the presence or absence of free cap analog competitor.
7G cap using [α-32P] - GTP and guanylyltransferase (see experimental procedures for details).
Xenopus elongation factor 1α gene was in vitro transcribed (Megascript, Ambion) and capped using vaccinia virus guanylyltransferase and [α -