Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Plural form of hexamer.

Etymologies

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Examples

  • RNA was quantified using a Nanodrop spectrophotometer (Thermo Scientific). 1 µg of RNA was DNase-treated (Promega) and used in reverse transcription reactions primed with random hexamers (Superscript III, Invitrogen). cDNA samples were treated with RNase H and 5 µL of each sample were used for quantitative real-time PCR reactions.

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  • Briefly, equal concentrations of RNA from each sample was reverse transcribed in the presence of 1X reaction buffer which included dNTPs, random hexamers, oligo (dT), MgCl

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  • At the top of the page is the histogram of the distribution of - lnp values for all possible hexamers and at the bottom, the sorted list of hexamers that can be mapped on deregulated miRNAs with corresponding p-values.

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  • After the input gene lists have been determined, we proceed to compare the distributions of all possible hexamers on the 3′UTR sequences between them.

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  • We investigated whether it is possible to distinguish between similar miRNAs by using the p-values of flanking hexamers 1 and 3.

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  • The option to use hexamers conserved between human and mouse provides a refinement of results for processes and miRNAs that are conserved between the two species.

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  • The optimal weights combination for hexamers 1 and 3 were identified as 0.6 and 0 respectively.

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  • Non-overlapping six nucleotide long motifs (hexamers) are counted on the 3′UTR sequence of protein coding genes provided by Ensembl.

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  • This approach calculates frequencies for hexamers 1, 2 and 3 as well as

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  • Following the link "View Results per microRNA" the user is taken to a page (B) showing miRNAs sorted according to a combinatorial score produced by the values of hexamers 1 and 2.

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