from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. Any enzyme that catalyzes the addition or removal of the elements of water; a hydrase
The interconversion of citrate and isocitrate by aconitate hydratase was shown to occur with complete retention of the proton transferred between C2and C3 but with no retention of the OH - between C3 and C2.
This did not seem such a formidable task, although it was not until 1963 that Kenneth Hanson and I solved the historically important problem of the prochirality of citric acid7, which was necessary for me to gain a proper perspective on the aconitate hydratase reaction.
These include subunits of pyruvate synthase, aconitate hydratase, and succinyl-CoA synthetase.