hyg

So the experience of death is turned into that of the exchange of functionaries, and anything in the natural relationship to death that is not wholly absorbed into the social one is turned over to hyg

Think Progress » Bolton Nomination Could Undermine Peace in Asia 2005

Chr. 17, chromosome 17; hyg, hygromycin resistance gene; neoSD, neo splice donor; SA neo, splice acceptor neo; puro, puromycin resistance gene.

Naturejobs - All Jobs Deniz Simsek 2010

Hygromycin was then shuttled from the linearized hygromycin marker (Clontech) to make pIRES-hyg-pcDNA3. 1z.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Next, the 1.2 kb fragment of rtTA driven by CMV promoter was digested with EcoR I and Hind III from plasmid pTet-on Advance vector (Clontech), blunt ended and ligated with BamH I linearized and blunt ended pIRES-hyg-pcDNA3. 1z to result in prtTA-IRES-hyg-pcDNA3. 1z.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

To construct pIRES-hyg-pcDNA3. 1z, IRES (internal ribosome entry site from virus EMCV) was digested from pBL-EP (kindly provided by Dr. John Olsen, University of North Carolina) by EcoR I and EcoR V and cloned into linearized pcDNA3. 1z vector.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Next, the 1.2 kb fragment of rtTA driven by CMV promoter was digested with EcoR I and Hind III from plasmid pTet-on Advance vector (Clontech), blunt ended and ligated with BamH I linearized and blunt ended pIRES-hyg-pcDNA3. 1z to result in prtTA-IRES-hyg-pcDNA3. 1z.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

To construct pIRES-hyg-pcDNA3. 1z, IRES (internal ribosome entry site from virus EMCV) was digested from pBL-EP (kindly provided by Dr. John Olsen, University of North Carolina) by EcoR I and EcoR V and cloned into linearized pcDNA3. 1z vector.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Hygromycin was then shuttled from the linearized hygromycin marker (Clontech) to make pIRES-hyg-pcDNA3. 1z.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

The regions used as porbes are indicated by solid lines with square ends while the expected bands are indicated by broken lines (with sizes indicated). hyg, hygromycin resistance gene from Streptomyces hygroscopicus doi: 10.1371/journal. pone.0006306.g004

PLoS ONE Alerts: New Articles Alistair K. Brown et al. 2009

The final delivery vector pOpp204 was generated by cloning the PacI cassette (hyg, p

PLoS ONE Alerts: New Articles Arunava Dasgupta et al. 2010