immunoblotted

Immunoprecipitates were incubated with or without active recombinant Cdc2/cyclin B and immunoblotted with phospho-raptor Ser696, Thr706 or total raptor.

PLoS ONE Alerts: New Articles Dana M. Gwinn et al. 2010

A549 cells were synchronized by double thymidine block and endogenous raptor was immunoprecipitated at the indicated times after release with an anti-raptor antibody and immunoblotted with phospho-raptor Thr706.

PLoS ONE Alerts: New Articles Dana M. Gwinn et al. 2010

Surface receptors were isolated by precipitation using Streptavidin-agarose beads and immunoblotted with the indicated antibodies.

Naturejobs - All Jobs Young Ho Suh 2010

HEK293T cells transiently expressing myc-tagged raptor were treated for 16 h with taxol and immunoprecipitates were immunoblotted with an antibody that recognizes phospho-threonine followed by proline.

PLoS ONE Alerts: New Articles Dana M. Gwinn et al. 2010

We loaded and immunoblotted 20 μg of protein from each subcellular fraction as indicated.

Naturejobs - All Jobs Young Ho Suh 2010

The membrane was also immunoblotted for GAPDH as a loading control.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Proteins in the hydrophilic and hydrophobic fractions were immunoblotted with anti-C-terminal-optineurin and anti-GM130.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Pulled down proteins were also immunoblotted with rabbit anti-C-terminal optineurin antibody (1: 2000) to confirm that optineurin protein was immunorecipitated.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Thereafter, optineurin-His was pulled down with rabbit polyclonal anti-His antibody, and the pull down was immunoblotted under reducing conditions with mouse monoclonal anti-FLAG.

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Equal volumes of lysate were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and immunoblotted for ZNF9 protein.

PLoS ONE Alerts: New Articles Morgan A. Sammons et al. 2010