Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • v. Simple past tense and past participle of immunoblot.
  • adj. Produced by immunoblotting

Etymologies

Sorry, no etymologies found.

Examples

  • Pulled down proteins were also immunoblotted with rabbit anti-C-terminal optineurin antibody (1: 2000) to confirm that optineurin protein was immunorecipitated.

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  • Proteins in the hydrophilic and hydrophobic fractions were immunoblotted with anti-C-terminal-optineurin and anti-GM130.

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  • Thereafter, optineurin-His was pulled down with rabbit polyclonal anti-His antibody, and the pull down was immunoblotted under reducing conditions with mouse monoclonal anti-FLAG.

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  • The membrane was also immunoblotted for GAPDH as a loading control.

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  • Surface receptors were isolated by precipitation using Streptavidin-agarose beads and immunoblotted with the indicated antibodies.

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  • We loaded and immunoblotted 20 μg of protein from each subcellular fraction as indicated.

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  • Equal volumes of lysate were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and immunoblotted for ZNF9 protein.

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  • HEK293T cells transiently expressing myc-tagged raptor were treated for 16 h with taxol and immunoprecipitates were immunoblotted with an antibody that recognizes phospho-threonine followed by proline.

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  • Immunoprecipitates were incubated with or without active recombinant Cdc2/cyclin B and immunoblotted with phospho-raptor Ser696, Thr706 or total raptor.

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  • A549 cells were synchronized by double thymidine block and endogenous raptor was immunoprecipitated at the indicated times after release with an anti-raptor antibody and immunoblotted with phospho-raptor Thr706.

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