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- noun Plural form of
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The immunocomplexes were washed 5 times with 800 µl of co-immunoprecipitation buffer, and immunoprecipitated proteins were released from the antibody-agarose-conjugate by the addition of 25 µl of glycine pH 2.5 with a 2 min incubation in ice.
To address this hypothesis, the cell lysates were firstly immunoprecipitated with anti-HIF-1α antibody, then subjected to a second immunoprecipitation with anti-bcl-2 antibody, and the immunocomplexes were analyzed by Western blot analysis using antibody against HSP90 protein.
In support of this hypothesis, we performed ChIP assay whereas chromatin immunocomplexes were immunoprecipitated with anti-HIPK2 and anti-Histone deacetylase
(Cell Signaling, Danvers, MA) overnight at 4°C, and then the immunocomplexes were adsorbed to protein A-Sepharose 4B (Amersham, Piscataway, NJ).