Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun A
protein precipitated duringimmunoprecipitation
Etymologies
from Wiktionary, Creative Commons Attribution/Share-Alike License
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Examples
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These experiments revealed that dMfn co-immunoprecipated with Parkin in wt flies, but failed to detect a dMfn band in an immunoprecipitate from Affinity purified anti-Parkin antiserum was used to immunoprecipitate Parkin from wt flies and park25 null mutants.
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We observed that in both human and mouse cardiomyocytes, BIN1 and Cav1. 2 colocalize at cardiac T-tubules (by fluorescence and electron microscopy immunogold labeling) and co-immunoprecipitate.
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The anti-ubiquitin antiserum detected a band of approximately 120 kDa in the immunoprecipitate from wt flies but detected only a very faint
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Affinity purified anti-dMfn antiserum was used to immunoprecipitate dMfn from wild-type flies,
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To control for the specificity of our anti-dMfn antiserum, we also immunoprecipated dMfn from flies expressing an RNAi construct targeting the dMfn transcript and subjected the immunoprecipitate to western blot analysis with an anti-dMfn and an anti-ubiquitin antiserum.
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In the course of testing whether a commercial rabbit antiserum to human Mitofusin 2 (Sigma (St. Louis, MO) #M6319) would cross-react with the corresponding fly protein, this antiserum was used to immunoprecipitate proteins from an adult fly protein extract and the immunoprecipitate was subjected to tandem mass spectrometry analysis.
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To help distinguish between these models we immunoprecipitated Parkin from wt flies and subjected the immunoprecipitate to western blot analysis with an anti-dMfn antiserum.
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As a control for specificity, anti-dMfn antiserum was also used to immunoprecipitate dMfn from flies with hsp70-GAL4 driven expression of UAS-dmfn-RNAiVienna.
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An anti-dMfn antiserum generated against the peptide DTVDKSGPGSPLSRF was provided by Dr. Alex Whitworth and used to immunoprecipitate dMfn from wt flies, PINK1 [B9] mutants, and park
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To control for the possible nonspecific interaction of dMfn with our anti-Parkin antiserum, we also subjected a protein extract from park25 null mutants to immunoprecipitation with an anti-Parkin antiserum and then used this immunoprecipitate in a western blot analysis with our anti-dMfn antiserum.
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