immunoprecipitates love

immunoprecipitates

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun Plural form of immunoprecipitate.

Etymologies

Sorry, no etymologies found.

Examples

  • PINK1 mutants and parkin mutants and subjected these immunoprecipitates to western blot analysis with an anti-dMfn and an anti-ubiquitin antiserum.

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  • By contrast, the abundance of ubiquitinated dMfn in the dMfn immunoprecipitates from

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  • MMP-2 immunoprecipitates were separated by SDS-PAGE and analyzed by autoradiography (A).

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  • In the middle and right panels, the dMfn immunoprecipitates derived using the anti-dMfn antiserum provided by Dr. Whitworth were subjected to western blot analysis with either anti-ubiquitin antiserum (middle panel) or our anti-dMfn antiserum (right panel).

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  • We report that dMfn is ubiquitinated in a PINK1 - and Parkin-dependent fashion, that the steady-state abundance of dMfn is inversely correlated to PINK1 and Parkin activity, and that Parkin co-immunoprecipitates with dMfn.

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  • (C) Raptor immunoprecipitates with endogenous cyclin B. Hela cells stably expressing myc-wt raptor with stable knockdown of endogenous raptor treated with or without taxol for 16 hours. myc-tagged raptor was immunoprecipitated and immunoblotted for Cyclin B.

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  • For all mass spectrometry (MS) experiments, myc-Raptor immunoprecipitates were separated using SDS-PAGE, the gel was stained with Coomassie blue, and the myc-Raptor band was excised.

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  • HEK293T cells transiently expressing myc-tagged raptor were treated for 16 h with taxol and immunoprecipitates were immunoblotted with an antibody that recognizes phospho-threonine followed by proline.

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  • Where indicated, immunoprecipitates were treated with or without calf-intestinal alkaline phosphatase (CIP) and then resolved in SDS-PAGE, and subjected to anti-myc immunoblotting.

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  • We further report that dMfn is ubiquitinated in a PINK1 - and Parkin-dependent fashion and that dMfn co-immunoprecipitates with Parkin.

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