immunoprecipitations love

immunoprecipitations

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Plural form of immunoprecipitation.

Etymologies

Sorry, no etymologies found.

Examples

  • Co immunoprecipitations (Co-IP) were performed on S2 cells co transfected with wild type Duf flag, Duf ΔCT1-flag, Duf ΔCT2-flag,

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  • Anti-myc immunoprecipitations were performed on cell lysates from HEK293T cells transiently transfected with myc-raptor treated with or without nocodazole.

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  • Anti-myc immunoprecipitations from HEK293T cells transiently transfected with myc-raptor for

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  • Anti-raptor used for endogenous raptor immunoprecipitations was from Invitrogen (#42-4000).

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  • For immunoprecipitations, cells were washed with ice cold PBS and collected in lysis buffer 1

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  • The 9E10 anti-myc antibody was used for immunoprecipitations (Roche).

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  • Three percent of the total amount of lysate used for the immunoprecipitations as well as the Parkin immunoprecipitates were subjected to western blot analysis with the anti-dMfn antiserum.

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  • In the left panel, 3% of the lysate input used in the immunoprecipitations was subjected to western blot analysis using an anti-ubiquitin antiserum to show that general ubiquitination levels were similar in all genotypes.

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  • To more carefully characterize PKA interactions in dendrites, we performed co-immunoprecipitations of AKAP5 and PKA with markers of the PSD and the major AKAP of the dendritic shaft, MAP2.

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  • Similarly, to identify proteins associating with endogenous PLN we carried out a second series of immunoprecipitations using the anti-PLN antibody 1D11 and fresh cardiac total muscle lysates.

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