immunopurified

The tryptic fragments were immunopurified with an anti-nitrotyrosine antibody and analyzed by MALDI-TOF mass spectrometry.

PLoS ONE Alerts: New Articles Alfredo Csibi et al. 2010

Flag-histidine-tagged CrPV-1A was immunopurified with anti-Flag antibody and analyzed by western blotting with

Naturejobs - All Jobs Arabinda Nayak 2010

An in vitro transcribed RNA was capped with guanylyltransferase using [α - 32P] GTP and then incubated with immunopurified intact gemin5 or the gemin5 (Δ25) deletion mutant in the presence or absence of free cap analog competitor.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

Crosslinking reactions (20 µl) contained 60 ng labeled RNA, 40 ng immunopurified protein or 120 µg cytoplasmic lysate, 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 1 mM MgCl

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

RNA was isolated from extracts (input) and from the immunopurified

PLoS ONE Alerts: New Articles 2008

Total RNA was isolated from cell extracts and immunopurified samples with the mirVana ™ PARIS ™ kit (Ambion).

PLoS ONE Alerts: New Articles 2008

+ and Arg II+ MØ were identified with a purified rat anti-mouse Mac3 monoclonal antibody (BD Biosciences), an immunopurified rabbit anti-mouse Arg II polyclonal antibody

PLoS ONE Alerts: New Articles Jamila Khallou-Laschet et al. 2010

[20], we tested whether human Tsc1 is also phosphorylated on Ser524, but could not detect any phosphorylation by mass spectroscopy with immunopurified hTsc1 from HEK293 cells (data not shown).

PLoS ONE Alerts: New Articles Sibylle Schleich et al. 2009

(50-100 ng) of the immunopurified RNAs were used for amplification. 8 µg of the amplified RNAs

PLoS ONE Alerts: New Articles 2008

Crucially, immunopurified gemin5 bound cap-sepharose in the absence of other proteins, and specifically crosslinked to radiolabeled cap-structure after UV irradiation (

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009