immunopurified love

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • adj. purified by immunopurification

Etymologies

Sorry, no etymologies found.

Examples

  • Flag-histidine-tagged CrPV-1A was immunopurified with anti-Flag antibody and analyzed by western blotting with

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  • The tryptic fragments were immunopurified with an anti-nitrotyrosine antibody and analyzed by MALDI-TOF mass spectrometry.

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  • Crosslinking reactions (20 µl) contained 60 ng labeled RNA, 40 ng immunopurified protein or 120 µg cytoplasmic lysate, 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 1 mM MgCl

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  • An in vitro transcribed RNA was capped with guanylyltransferase using [α - 32P] GTP and then incubated with immunopurified intact gemin5 or the gemin5 (Δ25) deletion mutant in the presence or absence of free cap analog competitor.

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  • RNA was isolated from extracts (input) and from the immunopurified

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  • Total RNA was isolated from cell extracts and immunopurified samples with the mirVana ™ PARIS ™ kit (Ambion).

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  • + and Arg II+ MØ were identified with a purified rat anti-mouse Mac3 monoclonal antibody (BD Biosciences), an immunopurified rabbit anti-mouse Arg II polyclonal antibody

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  • [20], we tested whether human Tsc1 is also phosphorylated on Ser524, but could not detect any phosphorylation by mass spectroscopy with immunopurified hTsc1 from HEK293 cells (data not shown).

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  • (50-100 ng) of the immunopurified RNAs were used for amplification. 8 µg of the amplified RNAs

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  • Crucially, immunopurified gemin5 bound cap-sepharose in the absence of other proteins, and specifically crosslinked to radiolabeled cap-structure after UV irradiation (

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