Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • adj. stained by means of immunostaining

Etymologies

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Examples

  • Then the images were taken using confocal laser microscopy (Zeiss). mRFP intensity in the immunostained embryo did not show significant difference from the unfixed embryo (data not shown).

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  • Right panels: B16-GFP and selected B16-GFP melanoma cells grown with high density C2C12 or 10T1/2 fibroblasts and immunostained for MiTF (red).

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  • Samples were fixed in 4%PFA and immunostained with anti-Oct4 and Alexa-488 conjugated donkey anti-mouse IgG to determine germ cells.

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  • Fifty µm sections were immunostained as described above.

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  • Frozen sections were immunostained for laminin to visualize the basal lamina of the fibers.

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  • RGC5 cells were double immunostained with anti-C-terminal optineurin (in green) and anti-GM130 (Golgi marker, in red).

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  • RGC5 cells were transfected for 20 h to express optineurin-GFP (OPTN-GFP, in green) and immunostained with anti-Rab8, anti-MyoVI, or anti-TfR. Cy3-labled secondary antibody was used and the Rab8, MyoVI and TfR.staining appeared in red (Cy3).

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  • Human RPE cells were immunostained with anti-C-terminal (Anti-C-term, in green) or anti-INT (Anti-INT, in green) optineurin along with anti-GM130 (in red).

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  • RGC5 cells were transfected for 20 h, fixed and immunostained.

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  • GAD65-GFP-positive cells immunostained with antibodies to Ki67, Mash1, GFAP or Olig2.

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