iodoacetamide

Samples were subjected to reduction with dithiothreitol, alkylation with iodoacetamide, and in-gel digestion with trypsin or chymotrypsin overnight at pH 8.3, followed by reversed-phase microcapillary/tandem mass spectrometry (LC/MS/MS).

PLoS ONE Alerts: New Articles Dana M. Gwinn et al. 2010

After completion of IEF, IPG strips were incubated for 15 min in equilibration solution I (50 mM Tris pH 6.8, 6 M urea, 30% glycerol, 2% SDS, 2% DTT, trace bromophenol blue) and 15 min in equilibration solution II (solution I with 2. 5% iodoacetamide instead of DTT).

PLoS ONE Alerts: New Articles Zhemin Zhou et al. 2010

Since iodoacetamide is a commonly used sulfhydryl agent, such a finding would suggest that the active-site Cys residue in

PLoS ONE Alerts: New Articles Yuan-Ping Pang et al. 2009

(IC50: 3.4 mM [64]) [64] imidazole reagents < 1, 5, 6 > [41] iodoacetamide

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On the other hand, it has been reported that iodoacetamide-containing AChE inhibitors do not bond covalently to

PLoS ONE Alerts: New Articles Yuan-Ping Pang et al. 2009

The strip is rehydrated for 8-12 hours with the application of 50 V at room temperature, then prepared for SDS-PAGE by incubation in reducing buffer (50 mM Tris, 6 M urea, 100 mM DTT, 30\% glycerol, and 2\% SDS) for 15 min then in alkylating buffer (50 mM Tris, 6 M urea, 250 mM iodoacetamide, 30\% glycerol, and 2\% SDS) for

PLoS ONE Alerts: New Articles Junnan Chen et al. 2008

The strip is rehydrated for 8-12 hours with the application of 50 V at room temperature, then prepared for SDS-PAGE by incubation in reducing buffer (50 mM Tris, 6 M urea, 100 mM DTT, 30\% glycerol, and 2\% SDS) for 15 min then in alkylating buffer (50 mM Tris, 6 M urea, 250 mM iodoacetamide, 30\% glycerol, and 2\% SDS) for

PLoS ONE Alerts: New Articles 2008

(at 1 mM 90% inhibition [10]) [10] iodoacetamide (at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects [6]) [6] Metals, ions Fe2+

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(substrate inactivates enzyme after a few turnovers [6]) [6] iodoacetamide

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[1] diethyldithiocarbamate [1, 2] iodoacetamide [1] p-hydroxymercuribenzoate

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