isopentane

Brains of mice, kept under DD conditions, were removed at indicated circadian times, then immediately frozen by isopentane, and stored at − 80°C until use.

PLoS ONE Alerts: New Articles Chihiro Kawaguchi et al. 2010

(Tissuetek) in isopentane that had been precooled in liquid nitrogen

PLoS ONE Alerts: New Articles Gemma L. Walmsley et al. 2010

Brains of mice, kept under DD conditions, were removed at indicated circadian times, then immediately frozen by isopentane, and stored at − 80°C until use.

PLoS ONE Alerts: New Articles Chihiro Kawaguchi et al. 2010

Brains of mice, kept under DD conditions, were removed at indicated circadian times, then immediately frozen by isopentane, and stored at − 80°C until use.

PLoS ONE Alerts: New Articles Chihiro Kawaguchi et al. 2010

For frozen sections, skin samples were embedded in HistoPrep (Fisher Scientific), solidified using dry ice-chilled isopentane and 5 µm sections were mounted onto Superfrost®/Plus slides (Fisher Scientific) as described

PLoS ONE Alerts: New Articles Tammy-Claire Troy et al. 2009

Spinal cord regions corresponding to T1-T5, T6-T12, and T13-L2 vertebrae were post-fixed overnight in 4% paraformaldehyde, flash frozen at − 65°C in isopentane (2-methyl butane), and stored at − 80°C.

PLoS ONE Alerts: New Articles Mitra J. Hooshmand et al. 2009

The produced fluid heats, through a heat exchanger, a secondary working fluid (isobutane, isopentane or ammonia), which vaporises at a lower temperature than water.

Recently Uploaded Slideshows 2009

After completion of experiments, animals were sacrificed by an overdose of urethane, the brains removed, frozen in isopentane at − 45°C and stored at − 80°C.

PLoS ONE Alerts: New Articles Jonathan Chetrit et al. 2009

The produced fluid heats, through a heat exchanger, a secondary working fluid (isobutane, isopentane or ammonia), which vaporises at a lower temperature than water.

Recently Uploaded Slideshows 2009

The produced fluid heats, through a heat exchanger, a secondary working fluid (isobutane, isopentane or ammonia), which vaporises at a lower temperature than water.

Recently Uploaded Slideshows 2009