latherin

F'r wan iv th 'rights guaranteed to his subjicks, be me sainted father, was a good latherin' ivry time it was comin 'to thim.'

Observations By Mr. Dooley Finley Peter Dunne 1901

Adj'tint Gen'ral; "I've put on my best coat to see you, Peg Barney," sez I. '"Thin take ut off again," sez Peg Barney, latherin' away wid the boot;

Soldiers Three Rudyard Kipling 1900

The volume fraction of latherin in the layer may thereby be estimated to be

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

Recombinant latherin (rlatherin) was produced without its hydrophobic secretory signal peptide and purified in high yield by over-expression in

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

The sequences of all the tryptic peptides obtained from sweat-derived latherin appear in the sequence encoded by the cDNA, as do those of horse allergens originally designated Equ c 4 and Equ c 5

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

Recombinant latherin was further purified by gel filtration (Superdex 75, 16 / 60, 50 mM Tris, 0.25 M NaCl, pH 8.4; Amersham Pharmacia / GE Bioscience, Chalfont St Giles, Buckinghamshire, UK). mRNA was extracted from equine tissues using Trizol treated with DNAase I (Ambion) to remove genomic material. cDNA was synthesised using Superscript II reverse transcriptase

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

In previous work [8] the surfactant properties of natural latherin had been characterized using a capillary method, and we found that recombinant latherin was similarly active; at approximately 1 mg ml

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

Five out of nine patients with detectable IgE antibody reactions to horse sweat exhibited IgE antibody to recombinant latherin.

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

The thermal stability of latherin in solution was investigated using a MicroCal VP-DSC

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

This interfacial layer seems surprisingly thin for a 23 kDa protein layer in equilibrium with the bulk solution where, judging from CD and DSC observations, latherin behaves as a folded globular protein.

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009