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- n. the N-lauroyl derivative of sarcosine; its sodium salt is an ionic detergent used to purify membrane proteins
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Barb and postdoctoral Fellow Brad Denker worked to develop a simple method for purifying the 28 kDa protein from the membranes of human red blood cells based upon its relative insolubility in N-lauroylsarcosine, a detergent that extracted the other membrane proteins.
The first incubation was performed in lysis buffer (0.5 M EDTA, 2\% N-lauroylsarcosine, 1 mg / mL proteinase K per ml) at 4°C for 20 to 24 h.