lipofectamine

Cells were washed with OPTI-MEM medium (Invitrogen) and were transfected using lipofectamine and Plus reagent (Invitrogen) and 100 ng of plasmid containing the cDNA coding for the mutated MMP-2, in OPTI-MEM.

PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010

Transient transfection of tumor cell lines using lipofectamine reagent

PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010

HEK-293 and fibroblast cells were grown on glass chamber culture slides (BD-Falcon) and transfected using lipofectamine (Invitrogen) following the manufacturers instructions.

PLoS ONE Alerts: New Articles Parveen Sharma et al. 2010

For the overexpression studies, U2OS cells were grown on glass coverslips and plasmid DNA was transfected with lipofectamine (Invitrogen life technologies) according to the manufacturer's protocol and cells were analyzed 24 h post transfection.

PLoS ONE Alerts: New Articles Jacqueline Schmuckli-Maurer et al. 2009

Briefly, empty vector or vector expressing the insulin receptor was transfected using lipofectamine

PLoS ONE Alerts: New Articles Siming Liu et al. 2009

DNA-lipofectamine complexes were added to the cells in DMEM without L-glutamine.

PLoS ONE Alerts: New Articles Felix Wussow et al. 2009

Two µg of A1 A2, A3, A4, A5 or A6 construct of the luciferase reporter gene and 20 ng internal control plasmid PhRL-sv40 were introduced into mouse lens epithelial αTN4-1 cells (Open bars) and human lens epithelial FHL124 cells (solid bars) in 12-well culture plates using lipofectamine 2000.

PLoS ONE Alerts: New Articles He-Ge Chen et al. 2009

For reporter gene activity, 2 µg of A1 A2, A3, A4, A5 or A6 construct of the luciferase reporter gene and 20 ng internal control plasmid PhRL-sv40 were introduced into mouse αTN4-1 cells, human FHL124 cells, or human ARPE-19 cells in 12-well culture plates using lipofectamine 2000.

PLoS ONE Alerts: New Articles He-Ge Chen et al. 2009

One to two µg of GFP-LC3B (rat) [26] was transfected into 2×10 5 cells using lipofectamine ™ 2000 according to the supplier's protocol (Invitrogen, CA).

PLoS ONE Alerts: New Articles 2008

1×105 HeLa per well of a 6-well plate were grown for 24 hours on glass cover slips and transfected with 1 µg plasmid DNA using 5 µl of lipofectamine (Invitrogen) according to the manufacturer's instructions.

PLoS ONE Alerts: New Articles 2008