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- n. A muralytic enzyme, obtained from Streptomyces, that is used in laboratories to degrade bacteria, and to isolate DNA
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For PCR amplification experiments, genomic DNA was isolated from GBS strains by mutanolysin-treatment of bacterial cells using a NucleoSpin Tissue kit (Macherey-Nagel), according to the manufacturer's instructions.
As reported previously, antisera against each protein did not show cross-reactivity with other pilin proteins Mid-exponential phase cells were harvested, washed in PBS and resuspended in 50 mM Tris-HCl, containing 400 units of mutanolysin (Sigma).