Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun Plural form of myotube.

Etymologies

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Examples

  • As observed in coculture with myoblasts, B16-GFP cells plated onto fully differentiated myotubes fuse with myotubes and do not secrete melanin (data not shown).

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • Myogenic conversion of B16-GFP and LLC1-GFP cells was quantified by determining the number of green myotubes expressing either MyoD or MF20, and expressing this as a percentage of the total number of myotubes per field (% of GFP+ myofibers). 10 randomly chosen fields in 3 independent experiments were analyzed.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • In addition, we observe that GFP labeled melanoma and Luis Lung carcinoma cells fuse to form myotubes when co-cultured with myoblasts.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • A similar frequency of GFP+ myotubes was observed in co-cultures of myogenic cells with the carcinoma cell line (LLC1) suggesting a general mechanism whereby myogenic cells inhibit expansion of metastatic tumors in skeletal muscle.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • GFP labeled carcinoma cells fuse with the C2C12 cells forming chimeric green myotubes, which are positive for myosin heavy chain (MF20, red) and the myogenic transcription factor MyoD (red).

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • Figure 2, we can identify myotubes (multinucleated cells) positive for GFP and MF20, indicating that GFP melanoma cells have fused with the differentiating myogenic cells.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • GFP labeled melanoma cells fuse with the C2C12 cells forming chimeric green myotubes, which are positive for MF20.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • Broadie KS, Bate M (1993) Development of larval muscle properties in the embryonic myotubes of Drosophila melanogaster.

    PLoS ONE Alerts: New Articles Sarada Bulchand et al. 2010

  • About 10% of myotubes in our co-cultures contained fused melanoma cells as evidenced by the presence of GFP suggesting that only a subpopulation of the melanoma cells undergo conversion.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

  • Staining of the myotube-like elongated B16-GFP cells with myogenic markers (MyoD and MF20) confirmed the fusion and incorporation of melanoma cells into myotubes.

    PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

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