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- adjective Subject to
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A simple and efficient protocol for production of highly pure and completely N-myristoylated Nef is presented.
Recombinant production of N-myristoylated proteins in
Importantly, stable overexpression of either FASN or myristoylated AKT (myr-AKT) was able to partially rescue cells from cell death induced by p63 silencing.
To this end, SCC9 and iPrEC cells were stably transfected with FASN, myristoylated AKT (Myr-AKT) or empty vector (EV). p63 silencing was achieved in the FASN overexpressing cells (FASN-SCC9 and FASN-iPrEC), Myr-AKT expressing cells (mAKT-SCC9 and mAKT-iPrEC) and control cells (EV-SCC9 and EV-iPrEC) by either transfection of siRNA or by generation of tetracycline-inducible clones expressing shRNA.