Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun medicine An antineoplastic compound methyl 5-(2-thenoyl)-2-benzimidazolecarbamate which inhibits the growth of microtubules

Etymologies

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Examples

  • In attempts to identify the residues of raptor responsible for the nocodazole-induced bandshift, microcapillary liquid-chromatography/tandem mass spectrometry (LC/MS/MS) was performed on raptor immunoprecipitated from cells with or without nocodazole treatment.

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  • To test whether Cdc2 phosphorylation of raptor might change the association of various components of mTORC1, epitope tagged cDNAs of the components of mTORC1 were expressed in HEK293E cells with or without nocodazole.

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  • Kinetic experiments, employing serum withdrawal, and stathmokinetic analysis with aphidicolin, mimosine or nocodazole demonstrated that cells with high levels of Mcm6* cycled with the committed phases of the cell cycle (S, G2, and M).

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  • Eight to ten hours later, 2 mM thymidine was added again for 14-16 hours, cells were washed twice with PBS, then released into thymidine-free media. 50 uM roscovitine was administered for 6 hours following 16 hours nocodazole treatment in A549 cells.

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  • Kinetic experiments, employing serum withdrawal, and stathmokinetic analysis with aphidicolin, mimosine or nocodazole demonstrated that cells with high levels of Mcm6* cycled with the committed phases of the cell cycle (S, G2, and M).

    BioMed Central - Latest articles

  • The microtubule was disrupted upon nocodazole treatment and the optineurin foci were dispersed from perinuclear region to distribute evenly in the cytoplasm.

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  • In some experiments, RGC5 and RPE cells transfected with pOPTN-EGFP for 20 h were untreated or treated with 10 µM nocodazole

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  • The cells were washed thoroughly to remove nocodazole (Washout) and allowed to recover for 1 h.

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  • (Control) or treated with 10 µM nocodazole for 30 min.

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  • We further examined cdc2 involvement through acute treatment of nocodazole arrested cells with the cdc2 inhibitor roscovitine.

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