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Examples
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At the times indicated, we collected total protein lysates (TCE), or fractionated separate samples into detergent-resistant (P3, chromatin) and detergent-sensitive (soluble / S1, cytosolic / nucleosolic) extracts
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The detergent-resistant pellets are operationally defined as chromatin-bound, while the detergent-soluble fraction contains nucleosolic and cytosolic proteins.
PLoS ONE Alerts: New Articles Piyali Mukherjee et al. 2009
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At the times indicated, we collected total protein lysates (TCE), or fractionated separate samples into detergent-resistant (P3, chromatin) and detergent-sensitive (soluble / S1, cytosolic / nucleosolic) extracts
PLoS ONE Alerts: New Articles Piyali Mukherjee et al. 2009
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B) In parallel with the BrdU-pulsed samples in A, MK cells were collected at the indicated times and separated into total cell lysates (TCE), or fractionated into nucleosolic / cytosolic detergent-soluble extracts (S1) or chromatin-bound detergent-resistant extracts (P3).
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B) In parallel with the BrdU-pulsed samples in A, MK cells were collected at the indicated times and separated into total cell lysates (TCE), or fractionated into nucleosolic / cytosolic detergent-soluble extracts (S1) or chromatin-bound detergent-resistant extracts (P3).
PLoS ONE Alerts: New Articles Piyali Mukherjee et al. 2009
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