peripherin

IKAP/hELP1 was expressed in the cytoplasm of peripherin expressing neurons.

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

Peripherin+ cells show high levels of IKAP/ELP1 (D and, indicated by arrowheads) in contrast to non-peripherin expressing cells in the culture (fig. 5D and E, indicated by asterisks).

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

Further evidence supports this view that most of the peripherin positive cells in our cultures express VAChT which is known to be present in cholinergic neurons of the PNS

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

(T) Quantitative analysis from these experiments (N-R) of the proportion of peripherin positive cells from total cells in the culture and the relative numbers of double stained Peripherin+/Brn3a+ and Peripherin+/Islet-1+ from total Peripherin+ cells.

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

Cross section of 5 weeks old NPs in suspension cultures expressing the migratory-crest marker HNK-1 throughout the sphere section (D), while at the edge of the sphere section colocalization of the PNS neuronal marker peripherin and the pan neuronal marker β-Tubulin III is observed (C, F and G respectively).

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

We believe that this observation together with the expression of crest specific markers can explains the commitment of our differentiated hNPs to yield a high proportion of peripherin expressing neurons following a total of 10 weeks in culture.

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

The vast majority of neurons that express peripherin also express the synaptic vesicle marker SV2 (

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

In order to further characterize these differentiated PNS neurons we performed double labeling immunofluorescence analysis using combinations of peripherin with Brn3a antibodies (

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

Further analysis of the peripherin positive population in these cultures revealed that only few (3-5%) peripherin positive neurons coexpress tyrosine hydroxylase (TH) (

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010

Figure 5C-E, indicated by arrowheads) in contrast to non-peripherin expressing cells in the culture (Figure 5C-E, indicated by asterisks).

PLoS ONE Alerts: New Articles Moran Valensi-Kurtz et al. 2010