from Wiktionary, Creative Commons Attribution/Share-Alike License
- v. Simple past tense and past participle of permeablise.
Sorry, no etymologies found.
SYTOX-Orange binds to the DNA of permeabilised parasites and is therefore used as a marker to generate death curves in the cytotoxicity assay (life-dead assay).
After stepwise re-hydration to PBS, samples were permeabilised with Triton-X100 (0. 2\% in PBS, then 0. 01\% in PBS, 10 min at room temperature).
Cells were fixed in 4% paraformaldehyde (Sigma) and permeabilised in 90% methanol.
Cells seeded on glass coverslips were fixed with 4\% paraformaldehyde: PBS and permeabilised with 0. 2\% Triton X-100: PBS as previously described
Cells were washed once with PBS, fixed with 4\% formaldehyde (in PBS) (Sigma #F8775) for 12 min, and permeabilised with 0. 5\% SDS in PBS for 10 min.
Cells were permeabilised for intracellular staining with cytofix/cytoperm kit (Becton Dickinson) and then stained with anti-granzyme-K (GM6C3; Santa
Snap shots of a pre-screen show that a digitonin concentration of 500 µg/ml permeabilised the membrane and killed the parasites within 40 min (