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permeabilization

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. the process of making something, such as a membrane or cell wall, permeable - often through the use of surfactants

Etymologies

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Examples

  • Non-specific interactions were suppressed with 5% fetal bovine serum (FBS) in permeabilization buffer (0.2% tween-20, 0.5% Triton X-100 in PBS pH 7.0) for 30 minutes and then samples were incubated with primary antibodies anti-PLN 1D11 and anti-COP I in permeabilization buffer.

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  • The permeabilization of mitochondrial membranes is a decisive event in apoptosis or necrosis culminating in cell death.

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  • One fundamental mechanism by which such permeabilization events occur is the calcium-induced mitochondrial permeability transition.

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  • Mitochondrial inner and outer membrane permeabilization were systematically investigated in cancer cell mitochondria versus non-cancerous mitochondria.

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  • Hence, this method based on MOMP (mitochondrial outer membrane permeabilization) is an interesting screening tool, tailored for identifying Bcl-2 antagonists with selective toxicity profile against cancer cell mitochondria but devoid of toxicity against healthy mitochondria.

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  • Mitochondrial outer membrane permeabilization (MOMP) is often required for activation of the caspase proteases that cause apoptotic cell death.

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  • The presence of pore-like structures under TEM suggests that the cytotoxic mechanism may include permeabilization of extracellular and/or intracellular membranes

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  • HT61-induced cytoplasmic membrane permeabilization determined by the DiSC3 (5) assay.

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  • In brief, sections were first rehydrated, then microwaved in 0.01 M sodium citrate buffer, followed by permeabilization with

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  • Endogenous surface GluR1 (D) or GluR2 (E) was revealed by immunofluorescence labeling without permeabilization using specific extracellular AMPAR antibodies.

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