permeabilized

The bacteria contain a palette of mutations that render them particularly susceptible to plasmid replication, and have been chemically permeabilized for transfer of the DNA across the membrane and cell wall in a procedure known as transformation.

Archive 2005-10-01 2005

CCE cells were fixed with 4% paraformaldehyde for 10 min, washed, and permeabilized with methanol.

PLoS ONE Alerts: New Articles Tzu-Ching Chang et al. 2010

For confocal microscopy of the cryosections, nuclei of fixed and permeabilized cryosections were stained with DAPI.

PLoS ONE Alerts: New Articles Ulrike Naumann et al. 2010

Cells were fixed, permeabilized and stained with goat anti-mouse F (ab) 2 Alexa 488.

PLoS ONE Alerts: New Articles Ulrike Naumann et al. 2010

For confocal microscopy of the cryosections, nuclei of fixed and permeabilized cryosections were stained with DAPI.

PLoS ONE Alerts: New Articles Ulrike Naumann et al. 2010

Nuclear transport is a biological topic in which SPT measurements have given several quantitative results in both permeabilized

PLoS ONE Alerts: New Articles Francesco Cardarelli et al. 2010

Although the membrane-disrupting activity of NP4P was slight or undetectable, ASABF-alpha permeabilized S. aureus membranes with enhanced efficacy in the presence of NP4P.

BioMed Central - Latest articles 2010

The cells were fixed and permeabilized with Cytofix/Cytoperm ™ buffer (BD BioScience) for 20 minutes at 4°C and incubated with allophycocyanin anti-IFN-γ or corresponding isotype controls (eBioscience) for 20 minutes on ice.

PLoS ONE Alerts: New Articles Xiaoling Gao et al. 2010

Cells fixed at room temperature for 1 h in Histochoice were unmasked in Citrate Buffer solution for 20 sec in a microwave, permeabilized with 20 mM Tris-HCl, pH 7.6, 137 mM NaCl, and 0. 1% Tween 20 containing 5% skim milk for 30 min at room temperature (according to Hattori et al., ref. 6) and incubated with primary antibodies overnight at 4°C.

PLoS ONE Alerts: New Articles Mara Marongiu et al. 2010

At 3 hpi, cells were harvested, fixed in 0. 5% PFA, permeabilized in 0. 1%

PLoS ONE Alerts: New Articles Christopher R. Cotter et al. 2010