from Wiktionary, Creative Commons Attribution/Share-Alike License
- v. Present participle of permeabilize.
Sorry, no etymologies found.
Location of the primary antibody was revealed with Alexa Fluor 488-F (ab′) 2 goat anti-mouse IgG after fixing for 5 min in 4% paraformaldehyde and permeabilizing the cells with 0. 02% Tween20.
This ~63-kDa band also increases in the mitochondria-rich membrane fraction following treatment with valinomycin, which, unlike CCCP, depolarizes mitochondria by permeabilizing the membrane to K PINK1 selectively accumulates on depolarized mitochondria.
Internalized receptors were detected with Alexa488-conjugated secondary antibody after permeabilizing cells in methanol (− 20°C) for 2 min.
In this study, the expression level of VEGF, a well-established vascular permeabilizing agent was shown to be elevated in a time-dependent manner, and exhibited a unique dual-response profile, in a DENV-infected endothelial cell.
ROP1 signal from inside the rhoptries is extremely weak since the permeabilization condition used (100% ethanol at room temperature) is not particularly efficient at permeabilizing the rhoptry organelles.
BCL-2, for example, is overexpressed in many cancers and mops up pro-apoptotic proteins to prevent them from permeabilizing mitochondria and initiating cell death.
To detect surface localized TEMs, PFA-fixation was employed without permeabilizing the plasma membrane.