from Wiktionary, Creative Commons Attribution/Share-Alike License
- adj. Made permeable
Sorry, no etymologies found.
After fixation, cardiomyocytes were permeablized and blocked with 0. 5% Triton X-100 (Sigma) and 5% NGS in PBS for 1 h at room temperature.
For co-staining of Cav1. 2 and α-tubulin in HL-1 cells, after fixation, the cells were permeablized with 0. 1% Triton X-100 for 15 min and blocked with 5% NGS for 1 h.
(B) The cells were stimulated for the indicated time points, fixed with paraformaldehyde (PFA) and permeablized with ice-cold 90% methanol, followed by staining with Alexa 647 labeled antibodies against phospho-STAT5 (Y694).
However, fixed and permeablized cells exhibited positive staining as numerous intracellular puncta.
The cells were fixed, permeablized, and stained with a normal IgG control antibody