Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A toxic cyclic peptide obtained from the mushroom Amanita phalloides

Etymologies

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Examples

  • Colonized monolayers demonstrated a time-dependent series of changes: staining with labelled phalloidin identified accumulation of actin at the cell junctions; ZO-1 staining revealed a loss of Caco-2 tight junction integrity; and Hoechst staining showed condensation and fragmentation of nuclear material consistent with apoptosis.

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  • There is phalloidin, which acts at once, and there is phallin, which dissolves red corpuscles ....

    The Witchcraft of Shirley Jackson

  • (C) Immunocytochemistry of the cells at the margin of the scratch wound with rhodamine-conjugated phalloidin in the presence of 5 µg/ml mitomycin-C.

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  • Therefore, we assessed the effect of bFGF on remodeling of the actin cytoskeleton by immunostaining the cells with rhodamine-conjugated phalloidin.

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  • Slides were washed with PBS-Tween20 (0. 02%) and incubated for 30 min with Alexa Fluor 488-F (ab′) 2 goat anti-mouse IgG, Alexa Fluor 594 phalloidin (both Invitrogen) and DAPI.

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  • Red: Actin stained with Alexa Fluor 594 phalloidin; blue Nuclei stained with DAPI.

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  • Alexa488-labeled phalloidin was used to detect F-actin and Hoechst stain to label nuclei

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  • The needles do not colocalize with actin (labeled with phalloidin-Texas Red). doi: 10.1371/journal. pone.0009014.g002

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  • The horse dermal fibroblast (NBL-6) cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 488 conjugated to phalloidin (green fluorescence), and for DNA with the bis-benzimidazole dye Hoechst 33258

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  • Subsequently, monolayers were stained with rhodamine / phalloidin (Molecular Probes, Carlsbad, CA) to visualize actin.

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