phosphopeptide love

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Any peptide that incorporates a phosphate group as a result of phosphorylation.

Etymologies

Sorry, no etymologies found.

Examples

  • After fractionation, tryptic digest and phosphopeptide enrichment, online liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed as described in 'Materials and methods'.

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  • To investigate kinase cascades triggered by the TLR4 ligand lipopolysaccharide (LPS) on systems level, we performed a global, quantitative and kinetic analysis of the phosphoproteome of primary macrophages using stable isotope labelling with amino acids in cell culture, phosphopeptide enrichment and high-resolution mass spectrometry.

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  • Here, we combined SILAC, phosphopeptide enrichment and high-accuracy mass spectrometry to analyse the phosphoproteome changes in resting versus LPS-activated primary bone marrow-derived macrophages.

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  • Comparison of more than three conditions can be achieved by including a common reference lysate in several pools, which is used for calculation of phosphopeptide ratios.

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  • Right panel: The isolated Chk2 FHA domain: phosphopeptide complex, shown in the same orientation as the boxed region in the left panel.

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  • It is not technically possible to directly assay Plk1-dependent alterations in phosphopeptide-binding capacity of the Chk2 FHA domain within cells expressing wild-type or mutant 53BP1.

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  • Initially, the Agilent HPLC-Chip II is available in a phosphopeptide chip (for post-translational modifications), the large-capacity protein ID chip, and the ultra-high-capacity chip.

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  • The two-dimensional phosphopeptide map analysis suggested that faster dephosphorylation of R406W was due to

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  • "This is the first statistically validated phosphopeptide signature to diagnose any disease, much less cancer or lung cancer," added senior co-author Dr. Martin Steffen.

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  • "This is the first statistically validated phosphopeptide signature to diagnose any disease, much less cancer or lung cancer," explained senior co-author Martin Steffen, MD, PhD, an assistant professor of pathology and laboratory medicine at BUSM, and director, Proteomics Core Facility at BUSM.

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