phosphopeptide

To investigate kinase cascades triggered by the TLR4 ligand lipopolysaccharide (LPS) on systems level, we performed a global, quantitative and kinetic analysis of the phosphoproteome of primary macrophages using stable isotope labelling with amino acids in cell culture, phosphopeptide enrichment and high-resolution mass spectrometry.

Naturejobs - All Jobs Gabriele Weintz 2010

Right panel: The isolated Chk2 FHA domain: phosphopeptide complex, shown in the same orientation as the boxed region in the left panel.

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Comparison of more than three conditions can be achieved by including a common reference lysate in several pools, which is used for calculation of phosphopeptide ratios.

Naturejobs - All Jobs Gabriele Weintz 2010

After fractionation, tryptic digest and phosphopeptide enrichment, online liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed as described in 'Materials and methods'.

Naturejobs - All Jobs Gabriele Weintz 2010

It is not technically possible to directly assay Plk1-dependent alterations in phosphopeptide-binding capacity of the Chk2 FHA domain within cells expressing wild-type or mutant 53BP1.

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Here, we combined SILAC, phosphopeptide enrichment and high-accuracy mass spectrometry to analyse the phosphoproteome changes in resting versus LPS-activated primary bone marrow-derived macrophages.

Naturejobs - All Jobs Gabriele Weintz 2010

Initially, the Agilent HPLC-Chip II is available in a phosphopeptide chip (for post-translational modifications), the large-capacity protein ID chip, and the ultra-high-capacity chip.

unknown title 2009

Initially, the Agilent HPLC-Chip II is available in a phosphopeptide chip (for post-translational modifications), the large-capacity protein ID chip, and the ultra-high-capacity chip.

unknown title 2009

The two-dimensional phosphopeptide map analysis suggested that faster dephosphorylation of R406W was due to

Journal of Biological Chemistry current issue Yotsumoto, K., Saito, T., Asada, A., Oikawa, T., K 2009

Initially, the Agilent HPLC-Chip II is available in a phosphopeptide chip (for post-translational modifications), the large-capacity protein ID chip, and the ultra-high-capacity chip.

unknown title 2009