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phosphorimaging

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. phosphoimaging

Etymologies

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Examples

  • Analysis of kinase assay by SDS-PAGE followed by phosphorimaging showed the presence of labeled proteins at approximately 93 kDa and 43 kDa corresponding to GST-PknJ-FL and His

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  • Bound proteins were released by boiling the beads 8 min in 0.1 ml 1% SDS/PBS, and GFP-tagged GRASP-1 truncations were immunoprecipitated with a rabbit GFP antibody and analyzed by phosphorimaging as before Eluates were boiled in Laemmli sample buffer, resolved on a 7. 5% SDS-PAA gel, and silver-stained.

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  • The products were detected by autoradiography and quantitated by phosphorimaging.

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  • Transcripts were visualized by 6\% polyacrylamide gel containing 7 M urea in TBE buffer and quantitated by autoradiography and phosphorimaging.

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  • Transcripts were detected by autoradiography and quantitated by phosphorimaging.

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  • RNA levels were assessed using phosphorimaging techniques and Image Quant software (version dFMR1 protein levels are increased in heads and ovaries from ago2 mutant flies.

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