phosphothreonine

(D, E, F) 2 µg of in vitro phosphorylated native kinase along with its activation loop mutants (as indicated) were resolved on 10% SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-phosphothreonine antibody as described in experimental procedures.

PLoS ONE Alerts: New Articles Gunjan Arora et al. 2010

No immunoreactivity was observed with anti-phosphothreonine (not shown).

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

PBST at 1: 10,000 dilution, directed against phosphothreonine (Invitrogen).

PLoS ONE Alerts: New Articles Gunjan Arora et al. 2010

No immunoreactivity was observed with anti-phosphothreonine (not shown).

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Amino acid standards, phosphoserine (pSer), phosphothreonine (pThr) and phosphotyrosine (pTyr) were added in the radiolabeled sample and visualized by ninhydrin staining (left panel) prior to autoradiography (right panel).

PLoS ONE Alerts: New Articles Gunjan Arora et al. 2010

(relative to binding capacity) of a biotinylated phosphothreonine-oriented peptide library

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Fig. 1C), but not toward anti-phosphothreonine (data not shown).

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

The immunoblotting of purified proteins after in vitro kinase assays with anti-phosphothreonine antibodies displayed comparable phosphorylation (

PLoS ONE Alerts: New Articles Gunjan Arora et al. 2010

Figure 7G) [79] reveals that one of these sites, Ser-164, is in close proximity to the phosphopeptide-binding site, with its phosphorylation likely to disrupt ligand binding through electrostatic repulsion of the ligand phosphothreonine residue (

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Phosphorylation of the proteins was assessed by immunoblotting with mouse monoclonal anti-phosphoserine, anti-phosphothreonine (1: 2,000, Sigma) and anti-phosphotyrosine (1: 1,000,

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010