phosphothreonine love

phosphothreonine

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. The phosphorylated form of threonine.

Etymologies

from Wiktionary, Creative Commons Attribution/Share-Alike License

phospho- +‎ threonine

Examples

  • No immunoreactivity was observed with anti-phosphothreonine (not shown).

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  • (D, E, F) 2 µg of in vitro phosphorylated native kinase along with its activation loop mutants (as indicated) were resolved on 10% SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-phosphothreonine antibody as described in experimental procedures.

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  • PBST at 1: 10,000 dilution, directed against phosphothreonine (Invitrogen).

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  • Amino acid standards, phosphoserine (pSer), phosphothreonine (pThr) and phosphotyrosine (pTyr) were added in the radiolabeled sample and visualized by ninhydrin staining (left panel) prior to autoradiography (right panel).

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  • (relative to binding capacity) of a biotinylated phosphothreonine-oriented peptide library

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  • Phosphorylation of the proteins was assessed by immunoblotting with mouse monoclonal anti-phosphoserine, anti-phosphothreonine (1: 2,000, Sigma) and anti-phosphotyrosine (1: 1,000,

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  • Fig. 1C), but not toward anti-phosphothreonine (data not shown).

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  • The immunoblotting of purified proteins after in vitro kinase assays with anti-phosphothreonine antibodies displayed comparable phosphorylation (

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  • Figure 7G) [79] reveals that one of these sites, Ser-164, is in close proximity to the phosphopeptide-binding site, with its phosphorylation likely to disrupt ligand binding through electrostatic repulsion of the ligand phosphothreonine residue (

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