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- n. Any enzyme that catalyzes the transfer of a phosphate group (phosphorylation)
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This was achieved by disrupting the main sugar transport system, the so-called sugar phosphotransferase system (PTS).
In addition, a selectable marker gene nptII encoding neomycin phosphotransferase II has been introduced.
A neomycin phosphotransferase (neo) cassette was inserted to replace most of the
To create the KO mice a neomycin phosphotransferase (neo) cassette was inserted into BamH1 and BglII sites within the
In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived from an evolved strain (PB12) lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS -) but with capacity to grow on glucose.
(RND) (blue); phosphotransferase system (PTS) (black).
We identified a missense mutation in the N-acetylglucosamine-1-phosphate transferase gene (GNPTAB), which encodes the alpha and beta catalytic subunits of GlcNAc-phosphotransferase (GNPT [EC 126.96.36.199