Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun biochemistry Several histidine residues linked together (usually at least six)

Etymologies

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Examples

  • Anti-polyhistidine peroxidase conjugate antibody (Sigma Aldrich) was used at 1: 10000 dilution.

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  • Anti-polyhistidine peroxidase conjugate antibody (Sigma Aldrich) was used at 1: 10000 dilution.

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  • Expression and purification of polyhistidine-tagged proteins

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  • Antibody to polyhistidine (1: 1,000 dilution; Sigma) was used to distinguish exogenous proteins from endogenous proteins.

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  • The expression of recombinant proteins was analyzed by electrophoresis on a 15% (wt/vol) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition and western-blotting with mouse antibody to polyhistidine (1: 1,000 dilution; Sigma) and sheep antibody to mouse Alkaline phosphatase (1: 10,000 dilution; Sigma).

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  • Germany) and the polyhistidine-tagged protein was eluted with 1×elution buffer (50 mM NaH

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  • Mouse monoclonal antibody to polyhistidine (1: 1,000 dilution;

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