post-fixed

The cell pellets were post-fixed with 2% osmium tetroxide in sodium cacodylate buffer for 1 to

PLoS ONE Alerts: New Articles Yu-Sin Kim et al. 2010

CEM cells, untreated or treated with anti-Fas (30 or 60 min), were fixed in 2. 5% cacodylate-buffered (0.2 M, pH 7.2) glutaraldehyde for 20 min at RT and post-fixed in 1% OsO

PLoS ONE Alerts: New Articles Maurizio Sorice et al. 2010

Briefly, the fixed cells were then post-fixed in OsO4 (2% OsO4 + 1. 5% potassium ferrocyanide, Sigma) and stained en bloc with 1% tannic acid (Sigma), uranyl acetate (EM Science) before being dehydrated in ethanol, cleared in propyline oxide, and embedded in eponate 12 (Ted Pella Co.).

PLoS Biology: New Articles Ting-Ting Hong et al. 2010

Tissue for transmission electron microscopy (TEM) was placed rapidly in 1% glutaraldehyde, fixed for at least 1 h at room temperature, and post-fixed in osmium tetroxide, and then processed into plastic.

PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

Brains were removed, post-fixed overnight in perfusant, then cryoprotected in a 30% sucrose solution.

PLoS ONE Alerts: New Articles Pramod K. Dash et al. 2010

The coverslips or cells pellet were washed twice in phosphate buffer and then post-fixed in 1% aqueous OsO Depletion of either condensin I or II results in abnormal centromere structure.

PLoS ONE Alerts: New Articles Alexander Samoshkin et al. 2009

Spinal cord regions corresponding to T1-T5, T6-T12, and T13-L2 vertebrae were post-fixed overnight in 4% paraformaldehyde, flash frozen at − 65°C in isopentane (2-methyl butane), and stored at − 80°C.

PLoS ONE Alerts: New Articles Mitra J. Hooshmand et al. 2009

The inserts were post-fixed in 1% aqueous osmium tetroxide at 4°C for 1 h, and washed three times with 70% ethanol before being dehydrated through an ethanol series.

PLoS ONE Alerts: New Articles Ram Naresh et al. 2009

Samples and histological sections were post-fixed with 4% paraformaldehyde and acetylated by 0. 25% anhydride acetic solution in triethanolamine 0.1 M.

PLoS ONE Alerts: New Articles Pascal Lapébie et al. 2009

For scanning electron microscopy (SEM) and semi-thin sections specimens were fixed in 2. 5% glutaraldehyde in a mixture of 0.4 M cacodylate buffer and seawater (1: 4: 5; 1120 mOsm) and post-fixed in 2% OsO

PLoS ONE Alerts: New Articles Pascal Lapébie et al. 2009