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from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A secondary fixation of a histological sample, often using osmium tetroxide to fixate lipids.


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  • After postfixation 30 min. in 4\% paraformaldehyde / PBStween, the nuclei were stained with Dapi (1 µg / ml) during 15-30 min followed by several washes in PBStween.

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