preincubated

Serially diluted Notch1 monoclonal antibodies were preincubated with a fixed amount (0.5 µg/ml) of Eu-labeled Notch1 ECD-Fc for 2 hr with shaking, followed by addition to the DLL4 ligand-coated plates.

PLoS ONE Alerts: New Articles Miguel Aste-Amézaga et al. 2010

We then preincubated Jurkat cells with recombinant IL-1β (10 pg/ml).

PLoS ONE Alerts: New Articles Frances Mercer et al. 2010

Confocal time-lapse images of MDCK cells expressing GalT-CFP preincubated with 1 μM palmostatin B, or equivalent DMSO as a control, for 55 min before and after microinjecton of PalFar.

Naturejobs - All Jobs Frank J Dekker 2010

The radiolabeled probe (2,000 cpm/reaction) was added to the preincubated mixture and further incubated at room temperature for 15 min.

PLoS ONE Alerts: New Articles 2010

B. MSCs were preincubated with PKH26 before intravenous injection.

PLoS ONE Alerts: New Articles Shaomei Wang et al. 2010

(3. 3U for reaction; Alexis biochemicals) was preincubated for 15 minutes at RT with the peptide RMS-P3/RR or the peptide RMS-P3/AA as negative control at increasing concentrations.

PLoS ONE Alerts: New Articles Katarina Hajdin et al. 2010

Cells were preincubated in the presence (+) or absence (−) of

Naturejobs - All Jobs Shinichi Nishimura 2010

B. MSCs were preincubated with PKH26 before intravenous injection.

PLoS ONE Alerts: New Articles Shaomei Wang et al. 2010

Floating sections were first preincubated in a blocking solution (BS; 1% BSA, 2% horse serum, and 2% goat serum) containing 0. 3% triton X-100 and subsequently incubated with the primary mouse anti-thrombin receptor PAR-1 antibody (1: 300, Zymed Laboratories, Invitrogen) and rabbit anti-GFAP (1: 500, Dako) diluted in BS.

PLoS Biology: New Articles Marta Gómez-Gonzalo et al. 2010

The radiolabeled probe (2,000 cpm/reaction) was added to the preincubated mixture and further incubated at room temperature for 15 min.

PLoS ONE Alerts: New Articles Michiko M. Nakano et al. 2010