recombineering love

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A technique, modelled on natural homologous recombination, used to systematically modify DNA

Etymologies

From recombinogenic + engineering (Wiktionary)

Examples

  • By retrofitting EL350, an established 'recombineering' E. coli strain, with a tightly regulated copy of trfA we have produced a new strain, MW005, which combines recombineering capacity with the useful ability to transiently induce copy-number of oriV-equipped clones.

    BioMed Central - Latest articles

  • To eliminate these intervening DNA isolation and transformation steps we retrofitted EL350 with a PBAD-driven trfA gene generating strain MW005 that supports, independently, both recombineering and copy-number induction.

    BioMed Central - Latest articles

  • Escherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence of resident clones via recombineering.

    BioMed Central - Latest articles

  • This new strain has been used to both generate, via recombineering, a number of reporter gene fusions directly from pCC1FOS-based Caenorhabditis elegans genomic clones and to transiently induce copy-number of fosmid and BAC clones prior to DNA preparation.

    BioMed Central - Latest articles

  • By coupling these two steps in a single strain, use of MW005 will enable the more rapid recombineering-mediated production of recombinant clones in the yield and quality necessary for many downstream purposes.

    BioMed Central - Latest articles

  • BAC and fosmid clones are highly suitable for modification by recombineering but, because they are present at low (1-2) copies per cell, the DNA is difficult to isolate in high yield and purity.

    BioMed Central - Latest articles

  • Relevant experience in molecular biology and recombineering would be an advantage.

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  • Oversee project development through appropriate high throughput pipeline production of recombineering clones.

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  • Proof of this concept was provided using mouse models wherein the locus that codes for Cdk4, one of the important gate-keepers of the mammalian cell cycle machinery, was targeted by genetic recombineering

    PLoS ONE Alerts: New Articles

  • In the context of the recently introduced highly versatile and markerless recombineering techniques

    PLoS ONE Alerts: New Articles

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