Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Alternative form of Sepharose.

Etymologies

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Examples

  • The immune complex was captured by the addition of 100 µl Protein G sepharose.

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  • The cell lysate was pre-cleared by adding 100 µl of 50% Protein G sepharose bead slurry per 1 ml of cell lysate.

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  • The Protein G sepharose beads were washed with cold ChIP sonication buffer containing no protease inhibitors.

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  • Nuclear extracts from unstimulated U937 cells were incubated individually with antibodies against Sp1, Sp3 and PPARγ followed by mopping of the immuno-complex with Protein G sepharose beads.

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  • The sepharose beads were washed 3 times with 800 µl ice-cold RIPA buffer and resuspended in 60 µl 2× sample buffer, followed by elution through boiling.

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  • Protein concentration of the dialyzed nuclear extracts was determined by the Bradford method. 30 µg of the dialyzed nuclear extracts were incubated with 2 µg each of anti-Sp1, anti - Sp3 and anti-PPARγ antibodies along with Protein G sepharose beads for 4 hours at 4°C with gentle shaking.

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  • The immune complex was precipitated by incubation of the samples with Protein G sepharose equilibrated with salmon sperm DNA at

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  • The supernatant was collected in fresh tubes and pre cleared with Protein G sepharose equilibrated with salmon sperm DNA for 30 minutes at 4°C.

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  • The cell lysates were centrifuged at 14 K rpm, 4°C for 20 min. The supernatant containing recombinant protein was collected and incubated with glutathione sepharose 4B affinity resin (GE Healthcare Bio-Sciences) pre-equilibrated with buffer A.

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  • For the biofilm inhibition assay, rabbit antibodies were purified using protein G sepharose, according to the manufacturer's instructions (Ab Spin Trap, GE Healthcare) and tested for their ability to inhibit biofilm formation.

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