supermix

PCR was carried out using Platinum PCR supermix at 20, 25, and 30 cycles each consisting of 2 minute extension and 30 second 50 C annealing (gapdh) temperatures with the following primers: GAPDH

PLoS ONE Alerts: New Articles 2009

For PCR amplifications, 2 ul of immunoprecipitated sample or 1 ul of input chromatin was incubated at 94 C for 5 minutes, followed by addition of 23 ul of Platinum PCR supermix (Invitrogen #10790-020) with each primer at 400 nM. 37 and 42 cycles of PCR using 2 minute extension and 30 second annealing at 48 C (p16, p16upstr) or 55 C (GAPDH).

PLoS ONE Alerts: New Articles 2009

RNA from cells was extracted using Aurum Total RNA Mini Kit (Biorad, Hercules, CA). cDNA was produced using Superscript III First Strand cDNA synthesis kit (Invitrogen) and Real-Time PCR was performed using iQ SYBR green supermix (Biorad) and an Opticon 2 real time PCR machine (Biorad).

PLoS ONE Alerts: New Articles Tommy Noh et al. 2009

I go into people's supermix and steal the little jelly men.

All Updates @ Ultimate-Guitar.Com 2009

(Invitrogen) according to the manufacturer's instructions. cDNA was measured by quantitative PCR with Platinum SYBR Green qPCR supermix (Invitrogen) on the Rotor-Gene 6000 (Corbett Life Science) using primers,

PLoS ONE Alerts: New Articles Luke W. Meredith et al. 2009

Mane-A*10 status while samples positive for SSP2, SSP1, and SSP3 were considered PCR was performed on cDNA prepared from inoculum viral stock RNA or RNA extracted from the basal ganglia using the SIV gag KP9-specific primers forward 5′-CAC GCA GAA GAG AAA GTG AA-3′ and reverse 5′-GTT CCT CGA AT (AG) TC (GT) GAT CC-3′ using Platinum PCR supermix (Invitrogen, Carlsbad, CA) and the following cycle conditions:

PLoS ONE Alerts: New Articles Joseph L. Mankowski et al. 2008