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- n. Any of several isomeric tetramethyl derivatives of benzidine, but especially 3,3’,5,5’-tetramethylbenzidine that is a chromogenic substrate used in immunohistochemistry
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The ROCK activity is measured by spectrophotometric analysis of color changes of tetramethylbenzidine
The plate was washed to remove any unbound protein and reagents followed by the addition of the substrate, tetramethylbenzidine (TMB) solution.
Sections were processed for the histochemical demonstration of HRP with the tetramethylbenzidine method