Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A thermal cycler

Etymologies

Sorry, no etymologies found.

Examples

  • She's got a DNA "thermocycler" bought on eBay for $59, and an incubator made by combining a styrofoam box with a heating device meant for an iguana cage.

    In Attics and Closets, 'Biohackers'

  • Normally when a machine is working for me, like say a thermocycler, I find other experiments to start and/or finish.

    Archive 2009-04-01

  • However, unlike a thermocycler, the FPLC needs a babysitter, providing few opportunities longer than five minutes to step away.

    Archive 2009-04-01

  • A typical thermocycler, which automates the heating and cooling of samples samples held at top.

    Ars Technica

  • Both PCR reactions were performed on either the Mx3005p thermocycler (Stratagene) or the LC480 thermocycler (Roche).

    PLoS ONE Alerts: New Articles

  • This test was used in both POC laboratories on a two-block SmartCycler system (reaction volume: 25 µl), and in the core laboratory on either an Mx3005p thermocycler (Stratagene) or an LC480 thermocycler (Roche) (reaction volume: 50 µl). rtRT-PCR reactions were performed using the SuperScript III Platinum One-Step qRT-PCR kit

    PLoS ONE Alerts: New Articles

  • Comparative evaluation with the same protocol operated in an MX3005P thermocycler (Stratagene) showed a lower sensitivity for low copy number specimens, but no significant difference in term of specificity (data not shown).

    PLoS ONE Alerts: New Articles

  • The advent of real-time PCR couples a modified thermocycler with real-time kinetics, for a highly accurate method of quantitating the template DNA or RNA.

    GEN News Highlights

  • After DNase I treatment, 1 µg of RNA was used for cDNA synthesis using the TaqMan reverse transcription reagents (Applied Biosystems, Foster City, CA), followed by real-time PCR quantification using SYBR Green and an Applied Biosystems 7300 real-time thermocycler (Applied Biosystems).

    PLoS ONE Alerts: New Articles

  • To amplify a long nDNA fragment, the thermocycler profile included initial denaturation at 94°C for 1 min, 27 cycles for β-globin or 26 cycles for β-polymerase of 94°C 15 sec, 65°C 12 min, with final extension at 72°C for 10 min.

    PLoS ONE Alerts: New Articles

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