thiogalactopyranoside

The temperature was reduced to 16°C for 1 h at which time recombinant SpGH38 was induced by the addition of 0.2 mM isopropyl 1-β-D-thiogalactopyranoside and incubated for sixteen additional hours.

PLoS ONE Alerts: New Articles Michael D. L. Suits et al. 2010

The bacterial cultures were grown to an optical density of 0.6 and expression of recombinant proteins were induced by the addition of isopropyl-beta-D-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM for 4 hours at 37C.

PLoS ONE Alerts: New Articles 2009

Protein expression was induced by the addition of 0.1 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) to the culture media, and incubation overnight at 17°C.

PLoS ONE Alerts: New Articles Adi Tabib et al. 2009

E. coli was transformed targeted vector, and then we induced the protein of NAT-NGF or LBD-NGF with 1 mM isopropyl β-D-thiogalactopyranoside (IPTG) at 37°C for 5 h. we harvested the two recombinant proteins accumulating in the inclusion bodies from cell lysis by centrifugation and solubilized in 8 M urea containing 0. 4% (vol/vol) β-Mercaptoethanol.

PLoS ONE Alerts: New Articles Wenjie Sun et al. 2009

To over express TcTS, 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma) was added and growth was continued with constant agitation at 18°C, for 12-16 hs.

PLoS ONE Alerts: New Articles 2008

To over express TcTS, 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma) was added and growth was continued with constant agitation at 18°C, for 12-16 hs.

PLoS ONE Alerts: New Articles Laura Ratier et al. 2008