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- n. The pyranoside form of a thiogalactoside
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The temperature was reduced to 16°C for 1 h at which time recombinant SpGH38 was induced by the addition of 0.2 mM isopropyl 1-β-D-thiogalactopyranoside and incubated for sixteen additional hours.
The bacterial cultures were grown to an optical density of 0.6 and expression of recombinant proteins were induced by the addition of isopropyl-beta-D-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM for 4 hours at 37C.
Protein expression was induced by the addition of 0.1 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) to the culture media, and incubation overnight at 17°C.
E. coli was transformed targeted vector, and then we induced the protein of NAT-NGF or LBD-NGF with 1 mM isopropyl β-D-thiogalactopyranoside (IPTG) at 37°C for 5 h. we harvested the two recombinant proteins accumulating in the inclusion bodies from cell lysis by centrifugation and solubilized in 8 M urea containing 0. 4% (vol/vol) β-Mercaptoethanol.
To over express TcTS, 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma) was added and growth was continued with constant agitation at 18°C, for 12-16 hs.