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- n. A polyalcohol with the molecular formula C4H10O4, primarily used as an intermediate in the chemical synthesis of other compounds.
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Glycerol is the most common cryoprotectant, followed by sorbitol and erythritol, ribitol, threitol, and sucrose.
Efficient erythritol catabolism under conditions that promote threitol synthesis, coupled with preferential threitol biosynthesis, appear to be responsible for the accumulation of high concentrations of threitol (250 mM) without concomitant accumulation of erythritol.
Threitol 4-phosphate appears to be the preferred substrate of the sugar phosphatase (s), promoting threitol synthesis over that of erythritol.
In vitro experiments show that threitol is synthesized from erythrose 4-phosphate, a C4 intermediate in the PPP.
The accumulation of two cryoprotective polyols, sorbitol and threitol, is integral to the extraordinary cold-hardiness of this beetle.
The sections were then rinsed two times in PBST containing 10 mM dithio-threitol (DTT,